ABSTRACT
Objective: To investigate the anti-inflammatory effect of Rosae Rugosae Flos (RRF) and screen its bioactive components on nuclear factor-κappa B (NF-κB) inhibition by ultra-performance liquid chromatography coupled with quadrupole/time of flight mass spectrometry (UPLC-Q/TOF MS). Methods: The anti-inflammatory effect of RRF was assessed in Pseudomonas aeruginosa strain-induced acute lung infection mouse model. UPLC-Q/TOF MS coupled with NF-κB activity luciferase reporter assay system was applied to detect the potential anti-inflammatory components in RRF extract. Results: The administration of RRF extract could ameliorate the neutrophil infiltration and diminish the levels of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), and IL-8 in P. aeruginosa induced mice. Seven components were screened to have potential NF-κB inhibitory effects based on the bioactivity-integrated UPLC-Q/TOF assay system. Conclusion: RRF can contribute to the alleviation of inflammation in mice with acute lung infection induced by P. aeruginosa strain. Seven potentially active ingredients, such as gallic acid, 4-O-galloylquinic acid, methylgallate, centaureidin, strictinin, casuariin, and ellagic acid are found to have anti-inflammatory effects as NF-κB inhibitors.